The closing theme to the cartoon, performed by Bis, includes the lyrics Blossom, commander and the leader; Bubbles, she is the joy and the laughter; Buttercup, she's the toughest fighter. This offers a nutshell description of the three Powerpuff girls' personalities.

Blossom

Blossom (voiced by Cathy Cavadini) is a character in animated television series The Powerpuff Girls. She has long red hair, pink eyes, and dresses in pink with a red bow on the top of her head and a heart-shaped hairclip when seen from behind. The self-proclaimed leader of the three Powerpuff Girls, she is often seen as the most mature, level-headed and composed member of the group, although she can also be fussy, overbearing, vain, and overly analytical at times. She tends to "mother" Bubbles and Buttercup, and often tries to play peacemaker between the two if they fight (ironically, she is very quick to argue with Buttercup). One episode, "A Very Special Blossom", also showed a darker side to Blossom, when she resorted to looting. Her special superpower is "Ice Breath" as revealed in the episode "Ice Sore", though in the same episode she has been shown to breathe fire. In "Ice Sore", surprised by her new power, Bubbles and Buttercup become very jealous of their sister. Blossom also studies Chinese, and knows how to speak it to a certain degree.

Bubbles

Bubbles (voiced by Tara Strong in the series and Kath Soucie in the What-a-Cartoon! episodes) is a fictional character in animated television series The Powerpuff Girls, quite similar to Dee Dee from Dexter's Laboratory, both by appearance and personality. She is portrayed as having short blonde hair in two pigtails, light blue eyes, and dresses in light blue. The ending theme refers to her as "the joy and the laughter", meaning that she acts as the baby of the group (despite being the same age) and is defined by innocence, playfulness and gentle demeanor. She does, however, have a tendency to be na?ve, ditzy, submissive and sensitive, leading to her often being regarded, by friends and foes alike, as the group's weak link. However, she can become very independent and aggressive when pushed, and out of all the Powerpuff Girls she is the most feared by Mojo Jojo after having single-handedly taken him down in a fit of rage in one episode. Her special superpower is that she can speak many languages, including Spanish (speaking) and Japanese (both reading and speaking), and communicate with animals, such as cats, and most notably squirrels (as revealed in the episode "Fuzzy Logic" and again in "Stray Bullet"). She also has the Sonic Scream (although later other girls seem to be able to use a weaker version too).

Buttercup

Buttercup (voiced by Elizabeth Daily) is a fictional character in animated television series The Powerpuff Girls. She has short black hair in a flip, light green eyes, and dresses in light green. Referred to in the ending theme as "the toughest fighter", she is the group's tomboy and is easily enraged. Sometimes her aggression gets the better of her, making her reckless, stubborn and too aggressive. She possesses a mean and somewhat vindictive streak not shared by her sisters. She also showed a selfish side in the episode "Moral Decay," to which she knocked out everyone's teeth for money. She has shown a softer side in several episodes, however. For example, in the episode "Cover Up", she had a soft green blanket that she was obsessed with that she would hug that gave her the confidence to be a better fighter, and she is quite protective over her sisters. In the episode "Nuthin' Special", her special "ability" is to roll her tongue (Blossom and Bubbles cannot do this, and nor can anyone else in Townsville), she also can turn into a tornado/twister and suck up her opponents and pop them out of the end of her tornado/twister. She is the toughest and strongest of the three girls. In the episode "Speed Demon" it was also revealed that she can fly slightly faster than her sisters. In "The Rowdyruff Boys", she has a tremendous disdain for resorting to kissing the boys in order to eliminate them, though willingly kisses a boy ridden with which she feared to be "cooties" in a later episode. During the birth of the girls in "The Powerpuff Girls Movie", she was initially shown to look extremely happy with wide eyes and an open mouthed smile up to the point where, last of the three girls to be named, Professor Utonium said he would name her Buttercup "because it also begins with a B!". Once she heard this, Buttercup immediately pouted. It is also revealed in the movie that Buttercup inspired the other girls to use their powers to fight crime and the forces of evil after she knocks down a giant evil gorilla who caused her to lose her temper with one punch; ironically, she initially apologized profusely for her actions, thinking she would get in trouble with everyone.